Table 2 BB experimental design for optimizing the most significant variables influencing on biocellulose concentration produced by Komagataeibacter hansenii AS.5 using EDCFs as a carbon and nitrogen source.
Trials | X1 (Temp) | X1 (Temp) | X3 (EDCFs) | X1X2 | X1X3 | X2X3 | X12 | X22 | X32 | Cellulose conc. (g/L) |
|---|---|---|---|---|---|---|---|---|---|---|
1 | − 1 (13) | − 1 (13) | 0 (90) | 1 | 0 | 0 | 1 | 1 | 0 | 0.624 |
2 | 1 (23) | 1 (23) | 0 (90) | − 1 | 0 | 0 | 1 | 1 | 0 | 0.602 |
3 | − 1 (13) | − 1 (13) | 0 (90) | − 1 | 0 | 0 | 1 | 1 | 0 | 0.266 |
4 | 1 (23) | 1 (23) | 0 (90) | 1 | 0 | 0 | 1 | 1 | 0 | 1.98 |
5 | − 1 (13) | − 1 (13) | − 1 (70) | 0 | 1 | 0 | 1 | 0 | 1 | 0.356 |
6 | 1 (23) | 1 (23) | − 1 (70) | 0 | − 1 | 0 | 1 | 0 | 1 | 1.200 |
7 | − 1 (13) | − 1 (13) | 1 (110) | 0 | − 1 | 0 | 1 | 0 | 1 | 0.218 |
8 | 1 (23) | 1 (23) | 1 (110) | 0 | 1 | 0 | 1 | 0 | 1 | 0.934 |
9 | 0 (18) | 0 (18) | − 1 (70) | 0 | 0 | 1 | 0 | 1 | 1 | 1.04 |
10 | 0 (18) | 0 (18) | − 1 (70) | 0 | 0 | − 1 | 0 | 1 | 1 | 1.108 |
11 | 0 (18) | 0 (18) | 1 (110) | 0 | 0 | − 1 | 0 | 1 | 1 | 1.246 |
12 | 0 (18) | 0 (18) | 1 (110) | 0 | 0 | 1 | 0 | 1 | 1 | 0.324 |
13 | 0 (18) | 0 (18) | 0 (90) | 0 | 0 | 0 | 0 | 0 | 0 | 1.281 |
14 | 0 (18) | 0 (18) | 0 (90) | 0 | 0 | 0 | 0 | 0 | 0 | 1.284 |