Figure 5
From: Screening of potential immune-related genes expressed during sepsis using gene sequencing technology
tSNE cell lineage location plot and multi-group comparison of pivotal genes. (A): tSNE overall plot of mixed samples from sepsis. Green color indicates T cell lineage; Yellow color indicates mononuclear-macrophage cell linage; Blue color indicates NK cell lineage; Grey color indicates B cell lineage. (B): CD300E as a biomarker for myeloid cells (positive control). (C): CD3D as a biomarker for T cells (positive control). (D–I) showed tSNE plots of BCL9L, BCL11B, CD247, CD96, MAFG and SAMD3, respectively. It suggesting these 5 essential genes were predominantly in the T cell lineage of PBMC from sepsis, only MAFG was located in monocyte cell lines. (J–O) showed violin plots of BCL9L, BCL11B, CD247, CD96, MAFG and SAMD3 expression in different samples. Vertical ordinate indicated relative expression levels. The abscissa represents different sample: NC for normal control 1; NS for non-survival sample of sepsis; SIRS for systemic inflammatory response syndrome; SV for survival sample of sepsis. ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: p ≥ 0.05.
