Figure 4
TNF-α induced a strong upregulation of the complement pathway (a) Upregulation of several members of the complement cascade pathway was observed especially in AAV-TNF-α transduced eye cup tissue. Colored squares in columns on the left indicate significant gene expression changes with an BH adjusted P-value < 0.05. (b) C3 expression was highly upregulated in AAV-TNF-α treated retina and eye cup. (c) C3 protein was also upregulated by AAV-TNF-α in an independent mouse cohort at 6 weeks after AAV-TNF-α treatment and this upregulation was partially rescued by anti-TNF-α treatment with golimumab (**p < 0.01; *p < 0.05, 1-way ANOVA with Tukey post-hoc test; n = 3–6) as measured by ELISA.
