Figure 4

(A) Some selected transgenic lines were screened under hygromycin (100 mg/ml) selection media to evaluate putative transgenic lines accompanied with ThSyGII (CEMB-SIG2) on MS test tubes. Transgenic line 1–5 withstood hygromycin selection medium, survived and showed robust growth under selection medium. The line labelled with 6 is non-transgenic which could not tolerate hygromycin supplemented selection media (B). The Fig. 4B indicated control sugarcane plants without any selection media which showed maximum growth in the same conditions as provided to putative transgenic lines but without any inclusion of selection drug. The figure was cropped to enhance focus and to remove distractions in the figures. Different figures were taken in different numbers and in various groups but all shown plants in figures were captured at the same day to develop synchronizations in our data. The originally available plant figures were also provided in supplementary information file as figure S-4A & S-4B. (C). The juvenile sugarcane transgenic line (3 months old) positive for ThSyGII phenotypically shown better tillering and health. Red labels (1, 2, 3, 4, 5) represented the numbers of tillers developed in transgenic sugarcane lines. (D). The sugarcane plant shown in this diagram indicates the phenotypic behaviour of control sugarcane plant, Red labels (1, 2) in this picture are sufficed to indicate less number of tillers than transgenic sugarcane line. The cropped figure focused only two sugarcane lines including both transgenic and control while full field grown sugarcane was provided in supplementary information file as S-4C &S-4D.