Figure 2
From: Functional and molecular dissection of HCMV long non-coding RNAs
HCMV lncRNAs other than RNA5.0 are required for lytic replication in HFFs. (a) Schematic representation for generation of HCMV lncRNA-Mut virus. (b) Schematic representation of lytic infection analysis in HFFs using WT, lncRNA-Mut, and lncRNA-Rev viruses. (c) Immunoblot assay of viral proteins, which indicate the lytic replication phase (IE1/2, Immediate early; UL44, early; pp28, late). GAPDH served as a loading control. Images are representative of two independent experiments. (d) Cell-free viral particles from supernatant medium were titrated via limiting dilution assay. Data represents mean ± SEM, and the statistical significance was calculated by two-way ANOVA with Tukey’s multiple comparisons test (n = 3).
