Figure 3 | Scientific Reports

Figure 3

From: Mblk-1/E93, an ecdysone related-transcription factor, targets synaptic plasticity-related genes in the honey bee mushroom bodies

Figure 3

Mblk-1-binding motif analysis and luciferase assay. (A) Three Mblk-1-binding motifs identified with DREME. (B) Two Mblk-1-binding motifs identified with MEME. (C) pGL4.23 reporter vector construction used for the luciferase assay. (D) The results of the luciferase assay using reporter vectors containing motif 1: 6xGAGA or 6xGAGA with 4-bp spacer sequence (left panel) and motif 2: 6xGAAATTTT or motif 3: 6xATTTTGG sequence (right panel). Data represent means ± SD (n = 3). Significant differences are indicated by asterisks (*p < 0.05, **p < 0.01, ***p < 0.005, Tukey–Kramer test).

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