Figure 4

Single-cell RNA-seq data analysis using adult MBs and identification of Mblk-1 target gene candidates preferentially expressed in lKCs. (A) UMAP visualization of single-cell RNA-seq data obtained from adult honey bee MBs by Traniello et al.³⁶. Clusters 1, 2, 6, and 7 correspond to lKCs. (B) Violin plots of gene expression: Mblk-1, CaMKII, and pumilio homolog 2. (C) Heatmap of expression of the top 5 cluster marker genes in each cluster. Positive values (shown in red or yellow) indicate that the gene is more highly expressed in the cluster indicated than in other clusters. (D) Ratios of genes suggested to be marker genes for 3 of the 4 clusters that correspond to lKCs (light blue), those for 2 clusters (blue), and those for 1 cluster (dark blue), respectively. Numbers in parentheses represent the number of Mblk-1 target gene candidates. (E–G) In situ hybridization results for the 3 marker genes. (E) Ca²⁺/calmodulin-dependent protein kinase II (F) pumilio homolog 2 (G) CUGBP Elav-like family member 4. The upper panels show results using anti-sense RNA probes, whereas the lower panels show results using sense RNA probes. Schematic drawing of the expression pattern of each gene in the MB is shown at the bottom. MB, mushroom bodies; OL, optic lobes; AL, antennal lobes; lKCs, large-type KCs. Scale bar: 250 μm.