Figure 2
Infectivity and shedding profiles of SARS-CoV-2/Vic-01 in four independent species. (A, B) Comparative shedding outcomes in nasal and oral/mouth swabs in female hamsters followed over the time course. SARS-CoV-2 genomic RNA levels expressed as Log10 International Units (IU)/mL calibrated against the WHO International Standard for SARS-CoV-2 RNA (NIBSC# 20/146/Eng02) amplified with E gene primers in nasal and oral swabs monitored over the 28 day time-course. Qualitative microculture data using VeroE6/TMPRSS2 indicator cells shown in squares as green (no virus) and red (positive virus culture) at the times indicated. Red bars indicate positive antigen lateral flow device result where sampled. (C, D) Subgenomic (sg) RNA levels expressed as copies/mL in female hamsters over the acute period. (E, F) Genomic (g) and subgenomic RNA levels in faecal pellets recovered daily over the first 10 days in female hamsters. Comparable nasal and oral swab data are shown for the 3 NHP species: cynomolgus macaques (G, H), tamarins (I, J) and marmosets (K, L). Culture data are similarly indicated by red or green squares and lateral flow device result by red or green bars where sampled.
