Figure 7

Tokuyasu cryo sections of lung tissue. At day 2 in hamster lungs (S62), virions (white open arrowheads) were visualised (A) and associated with perinuclear replication organelles (dotted ellipses) (B). At 10 dpi (S65) SARS-CoV-2 virion-like particles were further identified in lung tissue (C) with evidence of possible budding (D). Differences in morphology between infected cells and cells without current infection are apparent in hamster S60 extending out to 28 dpi (E) with replication organelles visible in infected cells (bottom cell). (F) (S60) is a higher magnification of E showing the replication organelle and mitochondria (black open arrowheads) with evidence of disrupted cristae indicating more generalised disrupted cell ultrastructure. (G–I) cynomolgus macaques S48, S46, S45 at days 2, 10 and 28 respectively. Virions shown (day10) with evidence of disrupted mitochondria 28dpi (panel I). (J–L) membrane-associated virions identified in marmoset S76 2 dpi. (M, N), tamarin lung S112 at day 2 with evidence of virions associated with a replication organelle. (O) uninfected hamster lung (S90) displays long sections of endoplasmic reticulum (er) in the perinuclear region. Magnifications range from 6000 × to 25,000 × with all scale bars corresponding to 500 nm. Annotations correspond to white open arrowheads, virions; black open arrowheads, mitochondria; and dotted ellipses, replication organelle. White asterisk, back asterisk and er denote collagen fibrils, nucleus and endoplasmic reticulum respectively.