Figure 1

Optimization of the MXDA-based extracellular matrix (ECM) clearing media. (a) Changes in the size and transparency of the rat tail tendon (RTT) depending on the concentration of the MXDA. Right graphs show the densitometric quantification results. Scale bar, 100 μm. (b) Quantification of RTT transparency and (c) swelling percent in different MXDA concentrations N = 9 animals. Data spots were obtained from 1–3 different RTTs from individual animals. (d) Effects of MXDA (40%) with iodixanol (IDX) or sucrose on the transparency and size of RTT. Both mixtures (iMAX or sMAX) were adjusted to RI = 1.47. Scale bar, 100 μm. (e,f) Quantification of transparency (e) and swelling percent (f) in the mixtures, N = 6 animals. Data spots were obtained from 1–3 different RTTs from individual animals. Standard one-way ANOVA with Tukey’s multiple comparison test was used for multiple comparisons (b,e).