Figure 3
Label-free imaging of mouse brain in iMAX solution. (a) A 2-mm thick coronal slice of mouse brain is imaged with selective-plane illumination microscopy (SPIM) and processed with Amira software for 3D reconstruction. Boxed area with while dotted line is magnified and shown in the left panel. Single-plane images of the boxed areas with yellow dotted lines are shown at the bottom panels. Scale bar, 2 mm for upper panel and 200 μm for lower panel. (b) Development of mouse white matters during the 7 days, 14 days, and 8 weeks after birth as imaged by the label-free SPIM imaging. Lower panels are magnified images of corpus callosum region. Scale bars, 1 mm. (c) Quantification of grey matter/white matter (GM/WM) signal ratio. N = 8 each stage. T-test was used to analyze the difference between groups.
