Figure 4
iMAX-based 3D imaging of the mouse organs. (a) Kidney glomeruli capillaries are visualized with CM-DiI infusion. Scale bar, 100 μm for left panel and 20 μm for right panel. (b) Green fluorescence protein (GFP) signals in the hippocampus of Thy1-GFP mice. Nuclei are counter-stained with PI. Scale bar, 100 μm. (c). Imaging of whole Thy1-GFP mouse brain. Red fluorescence was derived from the blood vessels pre-labeled by CM-DiI infusion. Right panel shows the orthogonal view of the ventral brain regions. Scale bars, 1 mm. (d) Imaging of spinal cord in the vertebrae. For the bone clearing, de-calcification was executed prior to the iMAX immersion. The specimen are placed on the 2-mm grid paper. Fluorescence signals from CM-DiI and Thy1-GFP are detected in the middle portion of the specimen as marked by black box. Scale bar, 100 μm. All fluorescent images were acquired using a TCS SP8 confocal laser-scanning microscope, and processed with Las X software (a,b,d) or Imaris (c) for 3D visualization.
