Figure 2
NLSM setup. Ti:Sa—laser for TPEF imaging, Yb:KGW—laser for TPEF and THG imaging, BC—beam combiner, L1 and L2—lenses of 1:1 beam expander for recollimation, VNDF—variable neutral density filter, GSM—galvanometer-scanning mirrors, L3 and L4—lenses of 1:3.75 beam expander for imaging, MDM—main dichroic mirror (cut-off 700 nm), Obj.—microscopic objective 40 × 1.3, Sam.—sample, Con.—aspheric condenser lens, DM—dichroic mirrors reflective for THG (347 nm) and transmissive for Yb laser (1040 nm), F1—Hoya glass UV filter, peak transmission 340 nm, F2—bandpass filter 275–375 nm, L6—focusing lens, THG PMT—photomultiplier tube for THG signal, TL—tube lens, BS/M—beam splitter or mirror toggle, Cam.—camera, F—VIS filter 400–700 nm for autofluorescence or VIS + 570 nm long pass for Nile Red fluorescence, L5—focusing lens, TPEF PMT—photomultiplier tube for TPEF signal, AD/DA—acquisition card. The scheme was created in Microsoft Power Point 2016 (https://www.microsoft.com/en-us/microsoft-365/powerpoint).
