Figure 3
Label-free imaging of Phycomyces blakesleeanus hyphae from the exponential phase in SLM. (a) one THG slice; (b) 3D model built out of 23 THG slices 0.9 µm apart. The average laser power at sample plane was 23–26 mW. (c) Multimodal imaging: bright field (BF) (left), autoTPEF (middle) and THG (right) images of the same live unlabeled hypha. The hypha was plasmolyzed and the retracted plasma membrane is solely visible in the THG image. The average laser power at sample plane was 2.7 mW (TPEF) and 55 mW (THG). Color intensity bar for both, TPEF and THG signals: deep blue—the lowest signal, red the highest signal. All the images were taken with Zeiss 40 × 1.3 oil objective lens.
