Table 1 A comparison of the number and size of lipid droplets obtained by the quantification analysis of the two methods, ICS and PSA.

From: Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi

Control/Treatment

Image correlation spectroscopy

Particles size analysis

Number of LDs/hyphae area (1/μm2) × 10−3 (mean ± SE)

Mean LDs diameter (μm) (mean ± SE)

Number of LDs/hyphae area (1/μm2) × 10−3 (mean ± SE)

Mean LDs diameter (μm) (mean ± SE)

Control cells for 3 h N-starvation

16 ± 1

0.56 ± 0.06

16 ± 2

0.74 ± 0.02

3 h N-starved cells

15 ± 5

0.60 ± 0.10

15 ± 6

0.74 ± 0.03

Control cells for 6 h N-starvation

15 ± 4

0.52 ± 0.08

13 ± 3

0.78 ± 0.02

6 h N-starved cells

24 ± 3

0.46 ± 0.02

22 ± 4

0.74 ± 0.04

  1. n = 3 for each group presented.