Figure 4

THBS1 is upregulated, but hsa-miR-29a-3p is downregulated, in PBMCs from severe patients, and THBS1 is targeted by hsa-miR-29a-3p. (a–c) Human PBMCs were isolated from mild (n = 8), severe (n = 7) patients and HCs (n = 8). THBS1 (a), IL17RA (b), and hsa-miR-29a-3p (c) expression levels were measured by qRT-PCR analysis. (d,e) THP-1 cells were transfected with either negative control (50 nM) or hsa-miR-29a-3p mimic (5, 20, 50 nM). After transfection, total RNA was extracted for the measurement of hsa-miR-29a-3p (d) and THBS1 (e) expression levels. Experiments were performed duplicate or triplicate and values are presented as means ± SEM (a–e). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Kruskal–Wallis test (a–c) and One-way ANOVA (d,e). NC negative control of hsa-miR-29a-3p mimic.