Figure 7
From: Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle
DIC and fluorescence microscopy of C. elegans L4 (a–d) and adult worms (e–g) after 24 h of treatment. (a,a’): Untreated control (M9 buffer with 1% DMSO) stained with Wheat Germ Agglutinin, Alexa Fluor 594 Conjugate (WGA). (b–d’) L4 larvae treated with CM (2 mg/mL) and subsequently stained with WGA (b’,c’) or fluorescence (λex 350/λem 430 nm) detected without staining (d’). Arrow indicates the partially shed cuticle strongly stained with WGA (c’). (e,e’) Untreated adult stained with WGA. Arrow indicates vulva, fluorescence within the worm corresponds to autofluorescence. (f,f’) Head region of CM-treated worm after WGA staining, showing strong fluorescence in the buccal capsule. (g) CM-treated adult worm stained with WGA. Scalebar 50 µm.
