Figure 3
From: Quantifying multiple stain distributions in bioimaging by hyperspectral X-ray tomography
Elemental segmentation of hindlimb by K-edge subtraction. (A) Elemental difference maps shown for the hyperspectral image slice shown in the sagittal plane (left). Colour bar for elemental difference maps is measured in terms of attenuation change, Δµ, as determined by KES of narrow energy windows (5 channels, ≈ 1.1 keV width total) either side of each absorption edge. (B) 3D volume mapping for elemental maps of iodine-, barium- and calcium-containing regions (top, middle, bottom respectively). (C) Virtual cross-section from a high spatial resolution XCT scan of the mouse hindlimb, showing the equivalent image slice following volume registration. Labels indicate the three material phases with expected stain uptake regions.
