Figure 4
From: Quantifying multiple stain distributions in bioimaging by hyperspectral X-ray tomography
Spectral analysis for a triple-stained forelimb specimen. (A) Elemental difference maps shown for the hyperspectral image slice shown in the coronal plane (left). Colour bar for elemental difference maps is measured in terms of attenuation change, Δµ. Arrows are included to highlight distinct soft tissue regions stained by each chemical, confirmed as hair (pink), vasculature (blue), skin (green) and muscle (white). (B) Reconstructed cross-section from a high spatial resolution DECT scan of the mouse forelimb, following basis material decomposition. The image slice matches that shown in the hyperspectral dataset, following volume registration. Labels indicate regions containing tungsten and calcium (green), as well as those containing iodine and barium (magenta). DECT decomposition fails to fully segment all elements into distinct phases. The dashed region and asterisk highlight the area below the elbow joint where PTA did not fully stain. (C) Reconstructed cross-section slice in the coronal plane of the triple-stained mouse forelimb. ROIs are shown for five distinct regions of the specimen, to analyse average voxel spectra. All ROIs cover 3 × 3 pixels, apart from ROI3, which covers a 2 × 2 region. (D) Voxel spectra for the ROIs selected in (C). Known K-edge positions are overlaid, with ROIs 1, 2 and 3 matching the K-edges for iodine, barium and tungsten respectively (top). Two ROIs, 4 and 5, show the presence of both iodine and tungsten contrast agents (bottom).
