Figure 1
HIF-1α and HIF-2α protein expression levels upon 1% O2 incubation. (a) HIF-1α and HIF-2α protein expression levels were quantified by western blot in H9 hESCs and FN2.1 hiPSCs upon 24 h of 1% O2 incubation. ACTIN was used as loading control. Representative blots of three independent experiments are shown (full-length or original western blot images are presented in Supplementary Fig. S8 and S9). Bar graphs represent the densitometric quantification of bands. Norm.: normoxia. Data are expressed as means + SEM fold induction relative to normoxia (arbitrarily set as 1) and Statistical analysis was done by Student’s t-test, (*) p < 0.05 and (***) p < 0.001 versus normoxia. (b) Analysis of mRNA expression levels of BNIP-3, BNIP-3L, and VEGF quantified by RT-qPCR in FN2.1 and H9 hPSCs at 24 h post 1% O2 treatment. RPL7 mRNA expression levels were used as normalizer. Graphs show mean + SEM mRNA fold induction relative to normoxia control cells (arbitrarily set as 1) of at least three independent experiments. Statistical analysis was done by Student’s t-test, (*) p < 0.05, (**) p < 0.01 and (***) p < 0.001 versus normoxia.
