Figure 4

NCBI-BLAST of genomic DNA amplified BBD129 gene from distinct fertility cross-bred cattle bulls. (A) The sequence variations were observed at the nucleotide level in the in the sequenced clones. Two polymorphisms were found in the exon-2 at 169th (T/G) and 329th (A/G) nucleotide positions. (B) The variations were also observed at the amino acids level. The BBD129 gene has shown two amino acid substitutions i.e., one at the 57th position (Serine to Alanine) and the 110th position (Asparagine to Serine).