Figure 1

Effects of PUFAs on activity of SARS-CoV-2 RdRp. (A) Recombinant RdRp was incubated with selected FAs at designated concentrations for 15 min at RT. Mix composed of NTPs and RNA template was then added, and reaction was carried out for 2 h at 34 °C. Signal was measured after 10 min at extension/emission = 488/535 nm with spectrofluorometer. Data are presented as % of control – 1.0% DMSO. (B) Recombinant RdRp or its mixture with accessory nsp7/nsp8 and RdRp overexpressed in VeroE6 cells was incubated with designated concentrations of LA for 15 min at RT. Mix composed of NTPs and RNA template was then added, and reaction was carried out for 2 h at 34 °C. Signal was measured after 10 min at extension/emission = 488/535 nm with spectrofluorometer. Data are presented as % of control – 1.0% DMSO, 100% inhibition – RdRp that was boiled at 100 °C and additionally exposed to UV for 30 min; insert – RFU readouts as an expression of activity of RdRp recombinant versus RdRp overexpressed. (C) Viability of cells treated only with designated concentrations of LA determined by measuring of absorbance after 24 h. (D) Viability of cells after treatment with designated concentrations of LA in the presence of viral particles determined by absorbance readouts after 24 h, EPA – eicosapentaenoic acid, LA – linoleic acid; ∆ p ≤ 0.01, * p ≤ 0.001.