Figure 4

Effect of intra-tracheal administration of LA against viral replication in vivo. (A) Pre-treatment effect of LA on HCoV-OC43 replication in lung tissue of K18-hACE2 C57BL/6 J mice. (B) Concurrent effect of LA on HCoV-OC43 replication in lung tissue of K18-hACE2 C57BL/6 J mice. (C) Post-treatment effect of LA on HCoV-OC43 replication in lung tissue of K18-hACE2 C57BL/6 J mice. (D) SARS-CoV-2 spike protein detection in mice lungs with western blot. (E) Quantification of SARS-CoV-2 spike protein performed as described in Material and Method section. Data are presented as mean + / − SD; uninfected untreated animal n = 3, uninfected treated with LA animal n = 3, infected untreated animal n = 8, infected treated animal n = 8; viral burden in lung tissue was determined by RT-qPCR and represented as viral copies normalized to mouse Pol2Ra; LA – linoleic acid; # p ≤ 0.05, ∆ p ≤ 0.01, * p ≤ 0.001.