Table 3 In silico analysis of mutant and wildtype c-kit gene of Acute Myeloid Leukemia patients (n = 31).
Tools | Parameters | Wildtype | Mutant | Expected |
|---|---|---|---|---|
Total number of amino acids | 976 | 976 | ||
Number of amino acids in model | 498 | 498 | ||
Swiss model | Template Query number | 2ec8.1.A | 2ec8.1.A | |
Sequence identity | 100 | 99.81 | ||
GMQE | 0.37 | 0.37 | ||
QMEAN | − 4.15 | − 4.22 | ||
Number of amino acids | 976 | 976 | ||
ProtParam | Molecular weight | 109,864.57 | 109,765.43 | |
Theoretical pI | 6.45 | 6.45 | ||
Helix | 6 | 6 (1%) | ||
Beta | 301 | 300 (60%) | ||
Coils | 191 | 192 (38%) | ||
Vadar analysis | Turns | 52 | 52 (10%) | |
Mean h-bond distance | 2.2 ± 0.3 | 2.2 ± 0.3 | 2.0 ± 0.4 | |
Mean h-bond energy | − 2.0 ± 0.9 | − 2.0 ± 0.9 | − 2.0 ± 0.8 | |
Number of residues with H bond | 285 (57%) | 285 (57%) | 373 (75%) | |
Z score | − 7.72 | − 7.73 | ||
ProSA web | Chain | A | A | |
Amino acids | 498 | 498 | ||
Percentage of all residues in favored regions | 88.9% (441/496) | 88.9% (441/496) | ||
Molprobity | Percentage of all residues in allowed region | 97.0% (481/496) | 97.0% (481/496) |
