Figure 4 | Scientific Reports

Figure 4

From: MKL-1-induced PINK1-AS overexpression contributes to the malignant progression of hepatocellular carcinoma via ALDOA-mediated glycolysis

Figure 4

MiR-34a-5p targets ALDOA to exert tumor suppressive effects in HCC. (a) Venn diagram showing the 2 miRNAs predicted by four different algorithms to target ALDOA. (b) RT-qPCR was used to detect the expression of miR-34a-5p and miR-122-5p in Huh-7 and Hepg2 cells. (c–f) WB detection of ALDOA protein expression levels after overexpression of miR-34a-5p and miR-122-5p. Statistical data are shown. (g) Kaplan–Meier survival curves showing the effect of miR-34a on overall survival. (h,i) Putative binding sequence of miR-34a-5p in the 3′UTR of ALDOA. (j) Luciferase reporter experiment revealed that miR-34a-5p could bind to the 3′UTR of ALDOA. (k,l) Enrichment of USP4 in HCC cells transfected with miR-34a-5p-WT and miR-34a-5p-MUT plasmids. (m) Luciferase reporter experiment on 293 cells co-transfected with miR-34a-5p mimic or mimic-NC and ALDOA-3′UTR-Luc-WT plasmid, with or without ALDOA. *P < 0.05; **P < 0.01; ***P < 0.001.

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