Figure 5

Empagliflozin inhibits renal apoptosis post-I/R. (A) Left, Representative images of TUNEL staining in mouse kidney sections. Scale bars, 50 μm. Sham sham-operated, I/R ischemia/reperfusion, EMPA empagliflozin. TUNEL-positive cells were stained green, and nuclei were stained with DAPI (blue). (B) The ratio of TUNEL-positive cells. n = 5 per group. (C, D) Representative images of immunostaining for Bcl-2 (C) and Bax (D) in kidney sections after renal I/R injury. Each group, n = 5. Scale bars, 50 μm. (E–G) The analysis of Bcl-2 (E), Bax (F) and Bcl-2/Bax (G) protein expression in mouse kidneys post-I/R. Each group, n = 5. Data shown are the mean ± SD. Significant differences between groups were determined by one-way ANOVA (B, E–G).