Figure 3

Optogenetic inhibition of PV+ interneurons increases LFP responses to multiple whisker deflections. (A–C) LFP responses to subsequent deflections of the same whisker after 50 ms or 100 ms. (A) Sketch of the stimulation protocol. (B) Left: Average LFP responses to the three stimuli illustrated in the sketch, without light application. Arrows indicate LFP components representing the 2nd stimulus pulse. Right: As in the left panel, but during light application. Horizontal bars indicate period of light application. (C) Left: For 50 ms interpulse-interval, summation of the two LFP responses from whisker deflection alone and light application alone (gray) and when recorded under both tactile and light stimulation (red, same as in panel B). Right: Differences between the traces shown in the left panel. (D–F) LFP responses to subsequent deflections of adjacent whiskers (W2 50 ms after W1). D) Sketch of the stimulation protocol. (E) Left: Average LFP responses to the two stimuli without light. Right: As in the left panel, but with light application. (F) Left: For 50 ms interpulse-interval, summation of the two LFP responses from whisker deflection alone and light application alone (gray) and when recorded under both tactile and light stimulation (lilac, same as in panel E). Right: Differences between the traces shown in the left panel.