Figure 2

Messenger RNA (mRNA) expression of toll-like receptors (TLRs) associated with adaptor proteins on the intestinal mucosa of neonatal pigs. (A) The mRNA levels of myeloid differentiation factor 88 A (MyD88A). (B) The mRNA levels of myeloid differentiation factor 88 B (MyD88B). (C) The mRNA levels of toll/interleukin-1 receptor-domain-containing adaptor-inducing interferon-β (TRIF). The mRNA levels were determined using qPCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and beta-actin were used as an internal control to normalize changes in specific gene expressions. The results were presented as fold changes relative to the control animals. Different lower-case letters indicate significant differences between each group and each day post inoculation (DPI) (p < 0.05).