Figure 1
From: Protein biomarkers of cardiac remodeling and inflammation associated with HFpEF and incident events
Methodologic approach to analysis of HFpEF-associated proteins. In step 1 univariate models with FDR adjustment were used to test associations between individual Olink proteins and HFpEF cases and non-HF controls in the CATHGEN discovery cohort. In step 2, significant proteins were tested in a multivariate adjusted model. In step 3, GSEA, validation in the TECOS cohort and LASSO were used to determine relevant HFpEF-associated proteins. In step 4, discriminative capabilities of multi-protein models were tested, as well as prognostic models for clinical outcomes. Abbreviations: Body Mass Index (BMI), Diabetes Mellitus (DM), False Discovery Rate (FDR), Gene Set Enrichment Analysis (GSEA), Heart Failure (HF), Heart Failure with Preserved Ejection Fraction (HFpEF), Hospitalization (hosp), Jackson Heart Study (JHS), Systolic Blood Pressure (SBP), Trial Evaluating Cardiovascular Outcomes with Sitagliptin (TECOS).
