Figure 4
Transwell migration assay. (A1, 2) SEM photos from the migrated MDA-MB-231 cells from the lower side of the transwell membrane. These photos (200X, and 1000X) were taken 48 h after treatment the cells with AKBA, ABA, BA, and negative control (NC). (A3) Fluorescent imaging of red-labeled MDA-MB-231 cells that migrated to the lower side of the membrane. (B) Normalized inhibition in MDA-MB-231 cells migration obtained from quantitative analysis of SEM photos. *indicates a significant difference (P < 0.05) (N value = 3). (C) Normalized migration inhibition compared to the negative control, as obtained from the quantitative analysis of red-labeled cell migration (n = 3). (D) Relative gene expression of P53, P21, BAX, and BCL2 genes in MCF-7, MDA-MB-231, and MCF-10A cells after 24 h treatment with AKBA, ABA, and BA in comparison with the negative control. a, b, c, and d revealed different significant levels in each gene (N value = 3).
