Figure 5
From: Beneficial effects of SS-31 peptide on cardiac mitochondrial dysfunction in tafazzin knockdown mice
Respiratory chain supercomplex organization analysis. Isolated cardiac mitochondria were separated by BN-PAGE, transferred onto PVDF membrane, and subjected to western blotting analysis with specific antibodies, as indicated. Supercomplexes (SC4, SC3, SC2, and SC1) containing complex I and complex III in various amounts are shown. Complex I (Cx I) and complex III (Cx III) are also identified as monomers (A, D). Protein levels estimated by band densitometry (B, C, E, F) in TazKD and TazKD + SS-31 are expressed as percentages vs WT and TazKD + saline, respectively. Histograms G and H display the percentage of ADU of immune-revealed bands in free complexes and in supercomplexes in each lane. All histograms show the mean values ± SD (n = 4/group). Statistically significant differences are indicated: Student’s t-test (**p < 0.01, *p < 0.05).
