Figure 2
Spine density and composition on basal and apical dendrites of CA1 neurons in the hippocampus. (a) Photomicrograph of Golgi labeled cells of CA1 region in the mouse hippocampus (scale bar: 200 µm). (b) Larger view of panel a (scale bar: 200 μm). (c) Representative image of CA1 neuron (scale bar: 25 µm). (d) Representative examples of spine morphologies in CA1 (scale bar: 1 µm). (e) Density of spines on apical dendrites in CA1; data shown are mean ± SEM. Proportion of immature spines (f) and head spines (g) on apical dendrites in the CA1; data shown are median ± 95% CI. (h) Density of spines on basal dendrites in CA1; data shown are mean ± SEM. Proportion of immature spines (i) and head spines (j) on basal dendrites in the CA1; data shown are median ± 95% CI. Density data analyzed by One-way ANOVA. Composition data analyzed by Kruskal Wallis with Dunn’s post-test to negative control; *p < 0.05. n = 20–24 dendrites, from 6 neurons, from 6 animals per group.
