Figure 4
Adipocyte size (area) and expression of browning genes among perirenal and subcutaneous adipose tissue. Findings from histological evaluation of adipocyte’s areas from each depot (n = 8 PRAT and n = 5 SCAT), using midpower field (200x). At least 10 random microphotographs were taken from each specimen, resulting in a mean number of 160 adipocytes for perirenal and 94 for subcutaneous adipose tissue observed per patient. (A) Comparison of relative frequencies of adipocytes along adipocyte size area intervals, between SCAT and PRAT Mann Whitney test was used for comparison among depots within each adipocyte size interval. Data is presented as mean ± SEM from each subject distribution of adipocytes by size interval (B) Comparison of mean adipocyte area among subcutaneous and perirenal adipose tissue depots, using Mann–Whitney test. Mean ± SEM are used to depict data. (C) comparison of UCP1, DIO2 and PRDM16 gene expression between tumour-sided perirenal fat matched with subcutaneous adipose tissue samples; data presented as fold-change and SEM. *P < 0.05. PRAT, perirenal adipose tissue; SCAT, subcutaneous adipose tissue.
