Figure 5

Influence of MK-4 and MKH derivatives on ROT-induced ROS. The NIH/3T3 cells were treated with 3 µM MK-4, MKH-DMG, or MKH-SUC for 6 h in the presence of 10 µM ROT. Intracellular ROS levels were determined by DCFH-DA staining. The relative luminescence unit (RLU) obtained using the DCFH-DA reagent was standardized by RLU from the CTB assay as the living cell number. ***p < 0.001 versus vehicle group; ###p < 0.001 versus ROT only group (Tukey’s test). Mean ± SD (n = 3).