Figure 1 | Scientific Reports

Figure 1

From: Characterization of a new potent and long-lasting single chain peptide agonist of RXFP1 in cells and in vivo translational models

Figure 1

SA10SC-RLX binding to human RXFP1. Flow cytometry analysis (a) of two concentrations (11 and 100 nM) of Cy5-relaxin binding in wild type (wt) EA.hy926 cells (upper panel) or EA.hy926_RXFP1 cells (lower panel). The Y axis represents fluorescence intensity and the X axis the size of the particles. (a) is representative of 3 independent experiments with similar results. Concentration-dependent binding of Cy5-relaxin binding in wild type EA.hy926 cells (blue curve) or EA.hy926_RXFP1 cells (red curve) (mean ± SEM, n = 3 different experiments performed in duplicate) (b). Inhibition of 11 nM Cy5 relaxin binding by increasing concentration of unlabeled relaxin or SA10SC-RLX (mean ± SEM, n = 4 different experiments performed in duplicate) (c).

Back to article page