Figure 2
From: The transmembrane domains mediate oligomerization of the human ZIP4 transporter in vivo
The construction of Fluorescence Correlation Spectroscopy (FCS). Combining confocal microscopy with FCS to monitor protein dynamics in living cells, the plot represents the fluctuations in the detected fluorescence intensity arising from individual fluorescent molecules (the green dots within detection volume), and it is plotted as a function of time. During imaging (center panel), eGFP diffuses in (green) and out (blue) of the detection volume in both directions. Only eGFP located within the detection volume are measured at any given time.
