Figure 7
Comparison of detection performance amongst the five analytical methods for four possible knock-in events using CRISPR-cas9, the selection process for a promising F0 generation, and the designed strain in this study. (A) Based on results obtained from this study, the superiority of each method for detecting the mutation events is summarized. **More informative, *informative, and No Not informative or least information. (B) The flow for sorting a promising F0 generation is illustrated. Southern blotting was able to detect mosaicism and multicopies. The RAISING method was also capable of detecting multicopies. Conventional PCR and genome sequencing were unable to detect mosaicism and multicopies. (C) The flow for establishing a designed strain in the F1 generation is illustrated. Southern blotting identified multicopies and genomic rearrangements near the target knock-in allele. Droplet digital PCR could distinguish multicopies. Conventional PCR and genome sequencing were unable to detect multicopies.
