Table 1 Purification profiles of laccases from different white rot fungi.
Purification step | Volume (ml) | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification fold | Yield (%) | |
|---|---|---|---|---|---|---|---|
Trametes versicolor 11269 | Liquor | 150 | 283.4 | 1809.0 | 6.4 | 1.0 | 100 |
Dialysis | 30 | 136.8 | 1583.1 | 11.6 | 1.8 | 88 | |
Ion exchange | 8 | 3.8 | 800.0 | 211.8 | 33.2 | 44 | |
Gel filtration | 5 | 0.8 | 314.6 | 379.7 | 59.5 | 17 | |
Pleurotus ostreatus 1020 | Liquor | 150 | 125.3 | 1813.5 | 14.5 | 1.0 | 100 |
Dialysis | 30 | 103.9 | 1258.5 | 12.1 | 0.8 | 69 | |
Ion exchange | 8 | 7.8 | 769.8 | 98.8 | 6.8 | 42 | |
Gel filtration | 10 | 1.5 | 552.2 | 377.0 | 26.0 | 30 | |
Panus tigrinus 707 | Liquor | 150 | 167.0 | 3465.0 | 20.7 | 1.0 | 100 |
Dialysis | 30 | 128.7 | 3420.4 | 26.7 | 1.3 | 99 | |
Ion exchange | 8 | 6.9 | 1121.3 | 162.5 | 7.8 | 32 | |
Gel filtration | 10 | 1.5 | 552.4 | 375.8 | 18.1 | 16 | |
Lentinula edodes SC-495 | Liquor | 150 | 197.0 | 2295.0 | 11.6 | 1.0 | 100 |
Dialysis | 30 | 137.2 | 1137.6 | 8.3 | 0.7 | 50 | |
Ion exchange | 8 | 9.2 | 367.1 | 40.1 | 3.4 | 16 | |
Gel filtration | 10 | 2.5 | 295.4 | 118.2 | 10.1 | 13 |
