Figure 1
Cell-free protein synthesis of individually and coexpressed GABAB forming subunits: (A) Schematic Illustration of cell-free synthesis of the GPCR, GABAB, in a eukaryotic cell-free system (Sf21). (B) Autoradiograph showing 14C-labeled individual cell-free synthesis and equimolar coexpression of subunits 1 (BR1) and 2 (BR2). Samples were PNGase F (PNGF) treated (plus and minus) and arrows indicate the corresponding bands (BR1: 109.7 kDa, BR2: 107.2 kDa). (C) Detection of cell-free synthesized proteins with rabbit primary antibody targeting BR1 subunit by an equimolar enzyme-linked immunosorbent assay (ELISA). Individual and coexpression of BR1 and BR2 were applied (n = 3). (D) Detection of cell-free synthesized proteins using primary mouse antibodies targeting BR2 by an equimolar ELISA (n = 3). Individual and coexpression of BR1 and BR2 were applied (n = 3). A p-value of < 0.05 was considered statistically significant and indicated by *, while a p-value < 0.001 is indicated by ***.
