Figure 4 | Scientific Reports

Figure 4

From: KBTBD11, encoding a novel PPARγ target gene, is involved in NFATc1 proteolysis by interacting with HSC70 and HSP60

Figure 4

HSC70 and HSP60 affect KBTBD11 protein levels. (a) 3T3-L1 cells were infected with FLAG-His-tagged Kbtbd11 adenovirus. After 48 h of infection, incubated for 6 and 24 h with or without various VER, MZR, and MG132 concentrations, whole-cell lysates were prepared and subjected to western blot analysis using anti-FLAG, anti-HSC70, anti-HSP60, and β-actin antibodies. (b) Quantification of the relative KBTBD11, HSC70, and HSP60 protein levels detected by western blot. The data were normalized to β-actin. n = 3 per group, *p < 0.05, **p < 0.01 vs. DMSO control.

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