Figure 6 | Scientific Reports

Figure 6

From: KBTBD11, encoding a novel PPARγ target gene, is involved in NFATc1 proteolysis by interacting with HSC70 and HSP60

Figure 6

Schematic representation of the relationship between KBTBD11 and NFATc1 degradation in 3T3-L1 cells. During adipocyte differentiation, the increase in PPARγ is accompanied by an increased expression of the target gene, Kbtbd11. Next, Kbtbd11 is translated into KBTBD11, which binds to HSC70 and HSP60. The HSC70 and HSP60 binding to KBTBD11 is involved in the maintenance of the intracellular KBTBD11 protein levels and that of its target NFATc1 for proteolysis. (a) Under normal conditions, HSC70 and HSP6 maintain intracellular KBTBD11 protein homeostasis, which allows stable NFATc1 function. (b) The dissociation of the KBTBD11-HSC70 or KBTBD11-HSP60 complexes upon HSC70 and HSP6 inhibition might be responsible for the stability of KBTBD11 and increase the amount of KBTBD11 in the cell. As a result, the increased KBTBD11 ubiquitinates NFATc1 and promotes a decrease in intracellular NFATc1 levels.

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