Figure 1
From: A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3
Several Architectures of TL1A Ligand were Evaluated. (A) TL1A is a type I transmembrane (TM) protein. Ligand designs focused on the C-terminal TNF homology domain (TNFHD), which contributes to binding to DR3 and DcR3. The positions of two N-linked glycosylation sites and disulfide bonds are indicated. (B) Secondary structure of the TNF homology jellyroll domain, which features ten anti-parallel b-strands which fold into a b-sandwich structure. Ligand designs fell into three categories: (C) His-TL1A or His-scTL1A, (D) Fc-TL1A, Fc-scTL1A, or Fc-His-TL1A, and (E) HSA-TL1A or HSA-scTL1A. Note that the Fc + His-TL1A molecule was generated by co-transfection of His-TL1A with Fc-TL1A at a molar DNA ratio of 1:2, and the desired product was purified by protein A and Ni–NTA tandem affinity chromatography. Images were generated with Adobe Illustrator (Version 25): https://www.adobe.com/products/illustrator.html.
