Figure 6
From: A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3
Fc-TL1A-AKALFF was Immune to DcR3-Based Inhibition. T cells were treated with 0.1 mg of immobilized anti-CD3 antibody alone or in the presence of exogenous DcR3 at 10 mg/mL. Either Fc-scTL1A (red) or Fc-scTL1A AKALFF (blue) was used to co-stimulate T cells. Anti-CD28 Ab was used as a co-stimulatory positive control. Anti-CD3-activated T cells without co-stimulatory signal was used as negative control. IFNg levels were measured by MSD assay. Stastical significance was determined using an unpaired T-test for each construct and data represents averages from three independent experiments using a single T cell donor. Graphs were generated using Graphpad Prism (Version 9): https://www.graphpad.com/scientific-software/prism/.
