Table 2 ELISA results for TL1A constructs binding to DR3.
From: A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3
Molecule | Target oligomer | EC50 (nM): DR3 immobilized | EC50 (nM): TL1A immobilized |
|---|---|---|---|
His-TL1A (TL1W2) | Trimer | 6.8 ± 1.0 | 2.5 ± 1.0 |
His-scTL1A TL1W19 | Monomer | 27.3 ± 1.3 | N.B |
His-scTNFa | Monomer | N.B.* | N.B |
Fc-TL1A (TL1W3) | Hexamer | 12.4 ± 1.0 | 2.6 ± 1.1 |
Fc-His-TL1A (TL1W61) | Dimer | 10.2 ± 1.0 | 2.2 ± 1.1 |
Fc-scTL1A (TL1W14) | Dimer | 9.7 ± 1.0 | 1.5 ± 1.1 |
HSA-TL1A (TL1W9) | Trimer | 167.6 ± 1.1 | > 4,000 ± 283 |
HSA-scTL1A (TL1W15) | Monomer | 29.2 ± 1.2 | 3.8 ± 1.1 |
