Figure 6 | Scientific Reports

Figure 6

From: Intraoperative abobotulinumtoxinA alleviates pain after surgery and improves general wellness in a translational animal model

Figure 6

Iba1 immunolabelling in the lumbar spinal cord of saline- and aboBoNT-A- treated animals. Photomicrographs are taken from L5-L6 sections of representative animals that received a full-skin and muscle incision and retraction surgery (Day 0), followed by intraoperative treatment with either saline (A, B) or aboBoNT-A 400U (C, D) and tissue collection on Day 6. Insets in panels (B) and (D) depict Iba1 stained microglia in saline- (B) and aboBoNT-A-treated (D) animals. Panel (E) shows quantification of Iba1 immunolabelling in the foci with high intensity labelling (arrowhead) from lumbar (L) sections 1–2, 3–4, and 5–6 in representative animals (n = 3/group) treated with either saline (blue symbols) or aboBoNT-A (red symbols; n = 2 sections per animal) as well as their mean values (± SEM). Scale bar: 1 mm (A, C), 200 µm (B, D). **p < 0.01 vs saline-injected animals. Ionized calcium-binding adaptor protein-1, Iba1; AboBoNT-A, abobotulinumtoxinA; U, unit.

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