Table 5 The antioxidant activity of hemp and milk thistle seed oils determined by means of DPPH, ABTS, FRAP, CUPRAC assays (method/units); the different ways of oil sample extraction were taken into account is.
Oil | Simple extraction | SPE extraction | |||
|---|---|---|---|---|---|
Methanol | Ethanol | 80% Methanol | 80% Ethanol | Methanol | |
DPPH/IC50 [Vextract/Vmixture] | |||||
Hemp seed oil | 3.43 ± 0.02 | 3.39 ± 0.04 | - | 31.55 ± 0.60 | - |
Milk thistle seed oil | 5.28 ± 0.58 | 5.61 ± 0.74 | - | 28.96 ± 0.48 | - |
ABTS/% inhibition of the ABTS•+ radical cation | |||||
Hemp seed oil | 93.30 ± 1.10 | 90.12 ± 3.59 | 66.72 ± 1.02 | 61.65 ± 7.97 | 21.79 ± 4.96 |
Milk thistle seed oil | 79.59 ± 3.76 | 25.03 ± 4.24 | 44.72 ± 8.44 | 52.90 ± 2.09 | 67.19 ± 5.31 |
CUPRAC/CUPRAC units [µMTrolox /L extract] | |||||
Hemp seed oil | 132.56 ± 10.94 | 420.47 ± 5.86 | 98.71 ± 3.14 | 116.35 ± 1.77 | 56.47 ± 10.32 |
Milk thistle seed oil | 242.57 ± 28.04 | 255.48 ± 26.17 | 90.37 ± 8.74 | 128.32 ± 8.16 | 45.43 ± 7.00 |
FRAP/FRAP units [µM Fe2+ /L extract] | |||||
Hemp seed oil | 975.46 ± 90.58 | 1063.88 ± 39.23 | 487.04 ± 2.39 | 338.04 ± 18.35 | 318.53 ± 63.05 |
Milk thistle seed oil | 873.44 ± 40.75 | 2891.08 ± 270.04 | 75.52 ± 4.93 | 558.60 ± 13.84 | 107.88 ± 1.24 |