Figure 8
Migration and Invasion in neuroblastoma cells. (A), (B) Migration and invasion assays were performed with SK-N-AS KO cells (KO) and scrambled control cells (Scr-V) as described in Methods. Representative pictures from Boyden chambers of one migration and invasion experiment are shown in (A). Pictures from two additional migration and invasion experiments with SK-N-AS cells are shown in Supplementary Fig. 8C. (B) Densitometry analysis of migration and invasion density of scrambled and TRPM2 KO SK-N-AS cells. Three migration experiments and three invasion experiments were performed. The mean from each experiment was quantitated comparing KO to scrambled cells, and used to calculate mean + S.E.M. of all three. Statistical difference of SK-N-AS KO compared to scrambled control was analyzed by two-tailed T-test, **p < 0.003. (C) Schema of TRPM2 modulation of migration/invasion in neuroblastoma. Higher levels of TRPM2 enhance migration and invasion. In SH-SY5Y cells, increased migration and invasion were associated with increased expression of HIF-1α, E2F1, and FOXM1 transcription factors and downstream integrin targets, and activation of Akt and ERK.
