Table 3 Activity of the key enzymes of naphthalene and decane degradation.

From: Pseudomonas veronii strain 7–41 degrading medium-chain n-alkanes and polycyclic aromatic hydrocarbons

Substrate

Enzyme activity, μmol × mg−1 protein

Naphthalene 1.2-dioxygenase

Salicylate hydroxylase

Catechol 1.2-dioxygenase

Catechol 2.3-dioxygenase

Alkane hydroxylase

Naphthalene

41 ± 3 (36; 51)*

˂ 1 (36)

1002 ± 195 (51)

28 ± 8 (36; 51)

˂1 (36)

1053 ± 239 (51)

12 ± 5 (36; 51)

Succinate**

15 ± 3

˂ 1

8 ± 2

˂ 1

13 ± 5

Decane

23 ± 8 (50; 66)

˂ 1 (50; 66)

˂ 1 (50; 66)

˂ 1 (50; 66)

28 ± 2 (50; 66)

Decane and naphthalene

45 ± 5 (35; 40)

24 ± 3 (35)

˂ 1 (40)

˂ 1 (35; 40)

˂ 1 (35; 40)

24 ± 3 (35; 40)

  1. *Time, h.
  2. **Enzyme activity was measured in the biomass of cells sampled at the end of the exponential growth phase.
Back to article page