Figure 2 | Scientific Reports

Figure 2

From: Sex differences in the inflammatory response of the mouse DRG and its connection to pain in experimental autoimmune encephalomyelitis

Figure 2

Sex specific activation of the TNFα signaling pathway in vitro. (A) DRGs were harvested from male and female mice, immunopanned to enrich the proportion neuronal cells, treated for 1, 6 or 24 h with 1 ng/mL TNFα, then collected for western blotting. (B) There were no sex differences evident in JNK phosphorylation. (C) Male cells have greater P38 phosphorylation (D) Female cells have greater NFκB (P65 subunit) phosphorylation. Bars indicate mean (fold change of active over total protein of interest, normalized to total protein) ± standard error mean (SEM). Dashed line represents untreated condition mean n = 4 experiments. *P < 0.05, ***P < 0.001, sex factor, two-way ANOVA.

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