Table 6 Analysis of variance (ANOVA) of PBD for AuNPs biosynthesis, using the extracellular filtrate of Bacillus subtilis NRC1.

From: Optimization of Bacillus subtilis NRC1 growth conditions using response surface methodology for sustainable biosynthesis of gold nanoparticles

Source

Sum of squares

df

Mean square

F-value

p-value*

prob > F

Model

6.93

10

0.69

66.78

0.0027

A-casein hydrolysate

0.57

1

0.57

54.48

0.0051

B-dextrin

2.689E−003

1

2.689E−003

0.26

0.6458

C-initial pH

0.91

1

0.91

87.81

0.0026

D-incubation period

0.51

1

0.51

48.93

0.0060

E-inoculum size

3.600E−004

1

3.600E−004

0.03

0.8641

F-temperature

0.38

1

0.38

36.34

0.0092

AB

0.23

1

0.23

21.84

0.0185

AC

1.41

1

1.41

136.00

0.0014

AE

0.27

1

0.27

26.21

0.0144

BC

1.08

1

1.08

103.61

0.0020

Curvature

7.54

1

7.54

726.57

0.0001

Residual

0.03

3

0.01

  

Lack of fit

0.01

1

0.01

0.54

0.5373

Pure error

0.02

2

0.01

  

Cor total

14.50

14

   
  1. F value = 66.78; R2 = 0.99; Adjusted R2 = 0.98.
  2. *: Values of "prob > F" less than 0.05 indicate model terms are significant.